Each data set comprises a six-point, ten-fold dilution series, repeated in five independent runs, for two different amplicons: K1/K2, 102 bp, and K3/K2, 218 bp. Fluorescence readings were exported after background subtraction via baseline averaging of the 5 cycles immediately preceding the cycles in which fluorescence was first detected. Please read the Materials and Methods section of Rutledge et al. (2004) for more details.
Format
A tibble with 10,800 rows and 10 variables:
platePlate identifier. Because one plate (run) was used per dilution series,
platevalues are simply numbered 1 thru 5.wellWell identifier. Values are always
NA(not available). This variable is kept nevertheless to be coherent with other data sets from other similar R data packages.dyeThe type of dye used. In this data set the values are always
"SYBR", meaning SYBR Green I master mix (Roche).targetTarget identifier: the amplicon used, K1/K2 or K3/K2.
sample_typeSample type (all curves are standards, i.e.
"std").replicateReplicate identifier: 1 thru 4.
copiesStandard copy number.
dilutionDilution factor. Higher number means greater dilution.
cyclePCR cycle.
fluorRaw fluorescence values.
Examples
rutledge
#> # A tibble: 10,800 × 10
#> plate well dye target sample_type replicate copies dilution cycle fluor
#> <fct> <fct> <chr> <fct> <fct> <int> <int> <int> <dbl> <dbl>
#> 1 1 NA SYBR K1/K2 std 1 41700000 1 1 0
#> 2 1 NA SYBR K1/K2 std 1 41700000 1 2 0
#> 3 1 NA SYBR K1/K2 std 1 41700000 1 3 0
#> 4 1 NA SYBR K1/K2 std 1 41700000 1 4 0
#> 5 1 NA SYBR K1/K2 std 1 41700000 1 5 0.0007
#> 6 1 NA SYBR K1/K2 std 1 41700000 1 6 0.0022
#> 7 1 NA SYBR K1/K2 std 1 41700000 1 7 0.0005
#> 8 1 NA SYBR K1/K2 std 1 41700000 1 8 0.0047
#> 9 1 NA SYBR K1/K2 std 1 41700000 1 9 0.0107
#> 10 1 NA SYBR K1/K2 std 1 41700000 1 10 0.0203
#> # ℹ 10,790 more rows