This data set is for a set of quantitative real-time PCR runs that were performed in the presence of an optimal amplification reaction mix added with serial dilutions of IgG (0.0, 0.25, 0.50, 1.0, and 2.0 \(\mu g/ml\)) thus acting as the inhibitory agent. There are two replicates for each concentration of IgG. The concentration of the amplicon ND1/ND2 is 41,700,000 copies. Please read the Methods section of Guescini et al. (2008) for more details.
Format
A tibble with 400 rows and 10 variables:
platePlate identifier.
wellWell identifier. Values are always
NA(not available). This variable is kept nevertheless to be coherent with other data sets from other similar R data packages.dyeThe type of dye used. In this data set the values are always
"SYBR", meaning SYBR Green I master mix (Roche).targetTarget identifier: the amplicon used,
"MT_ND1".sample_typeSample type (all curves are standards, i.e.
"std").replicateReplicate identifier: 1 thru 3.
IgG_concIgG concentration in \(\mu g/ml\).
copiesStandard copy number.
cyclePCR cycle.
fluorRaw fluorescence values.
Examples
IgG_inhibition
#> # A tibble: 400 × 10
#> plate well dye target sample_type replicate copies IgG_conc cycle fluor
#> <fct> <fct> <fct> <fct> <fct> <int> <int> <dbl> <int> <dbl>
#> 1 NA NA SYBR ND1/N… std 1 4.17e7 0 1 -6.28e-2
#> 2 NA NA SYBR ND1/N… std 1 4.17e7 0 2 3.24e-3
#> 3 NA NA SYBR ND1/N… std 1 4.17e7 0 3 -7.34e-5
#> 4 NA NA SYBR ND1/N… std 1 4.17e7 0 4 -3.87e-3
#> 5 NA NA SYBR ND1/N… std 1 4.17e7 0 5 2.61e-4
#> 6 NA NA SYBR ND1/N… std 1 4.17e7 0 6 4.45e-4
#> 7 NA NA SYBR ND1/N… std 1 4.17e7 0 7 3.84e-3
#> 8 NA NA SYBR ND1/N… std 1 4.17e7 0 8 1.43e-2
#> 9 NA NA SYBR ND1/N… std 1 4.17e7 0 9 1.84e-2
#> 10 NA NA SYBR ND1/N… std 1 4.17e7 0 10 7.47e-2
#> # ℹ 390 more rows