qPCR data set by Boggy et al. (2010)

The qPCR data set named s1 comprises a six-point, ten-fold dilution series, replicated in duplicates targeting an amplicon designed with a random sequence that minimizes secondary structure and off-target primer binding. Please read the Quantitative PCR data section, qPCR assays subsection of Boggy et al. (2010) for more details.

Format

A tibble with 480 rows and 10 variables:

plate: Plate identifier. For this data set it is always missing (NA).
well: Well identifier.
dye: The type of dye used. In this data set the values are always "SYTO 13", meaning 2 mM of the dsDNA dye SYTO-13 (Invitrogen, Carlsbad, CA).
target: Target identifier: the amplicon used, a random sequence dubbed "S1" by the original authors.
sample_type: Sample type (all curves are standards, i.e. "std").
replicate: Replicate identifier: either 1 or 2.
copies: Standard copy number.
dilution: Dilution factor. Higher number means greater dilution.
cycle: PCR cycle.
fluor: Raw fluorescence values.

Source

doi:10.1371/journal.pone.0012355

Examples

s1
#> # A tibble: 480 × 10
#>    plate well  dye     target sample_type replicate  copies dilution cycle fluor
#>    <fct> <chr> <fct>   <fct>  <fct>       <fct>       <int>    <int> <dbl> <dbl>
#>  1 NA    D8    SYTO 13 S1     std         1             5e8        1     1 0.198
#>  2 NA    D8    SYTO 13 S1     std         1             5e8        1     2 0.197
#>  3 NA    D8    SYTO 13 S1     std         1             5e8        1     3 0.197
#>  4 NA    D8    SYTO 13 S1     std         1             5e8        1     4 0.197
#>  5 NA    D8    SYTO 13 S1     std         1             5e8        1     5 0.201
#>  6 NA    D8    SYTO 13 S1     std         1             5e8        1     6 0.207
#>  7 NA    D8    SYTO 13 S1     std         1             5e8        1     7 0.221
#>  8 NA    D8    SYTO 13 S1     std         1             5e8        1     8 0.250
#>  9 NA    D8    SYTO 13 S1     std         1             5e8        1     9 0.303
#> 10 NA    D8    SYTO 13 S1     std         1             5e8        1    10 0.400
#> # ℹ 470 more rows